For each one of these good reasons, it is critical to do more research on how children fare when being raised by their grandparents.’.. A grandparent in the home buffers the drawbacks of single-parenthood Many studies show that children living in a single-parent family tend to do worse academically and receive much less intellectual stimulation than children living with married parents. Having a grandparent in the home, however, appears to buffer a few of these negative effects, according to a fresh Cornell University study. ‘When searching at children’s test scores, we find that kids who live with a single mom and a grandparent fare equally well as children living with married parents,’ says Rachel Dunifon, assistant professor of policy evaluation and management in the faculty of Human Ecology at Cornell.Brains were harvested after perfusion, and 10 percent homogenate was prepared in conversion buffer by adding protease inhibitors. To increase the performance of PMCA, 6 mM EDTA and 0.05 percent digitonin were added to the brain homogenate. Urine samples, processed seeing that described above, were mixed with 10 percent human brain homogenate in tubes containing 3 polytetrafluoroethylene beads and subjected to 96 cycles of PMCA with the use of a microsonicator . After one round of 96 PMCA cycles, an aliquot of the amplified material was diluted by a factor of 10 into fresh brain homogenate from transgenic mice and yet another circular of PMCA cycles was performed.